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| * '''Description:''' error prevention oxidized guanine system, confers protection against oxidative stress to vegetative cells, moreover the protein has RNA pyrophosphohydrolase activity <br/><br/>
| | {{#bank: MutTA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''mutTA''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ytkD'', ''rppH''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || 8-oxo-dGTPase (antimutator), RNA pyrophosphohydrolase
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || DNA repair, RNA degradation
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU30630 mutTA]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 18 kDa, 5.952
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 474 bp, 158 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ytlD]]'', ''[[ytkC]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU30630 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU30630 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU30630 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ytkD_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ytkD_3134983_3135459_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:mutTA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU30630]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[DNA repair/ recombination]]}},
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| {{SubtiWiki category|[[sporulation proteins]]}},
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| {{SubtiWiki category|[[RNases]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[SigF regulon]]}}
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|
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU30630
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU30630&redirect=T BSU30630]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ytkD.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13869]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** removal of pyrophosphate from the 5' end of mRNAs to make the RNA accessible for degradation by [[RNases]] {{PubMed|21925382}}
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| ** RppH requires at least two unpaired nucleotides at the 5' end of its RNA substrates and prefers three or more. The second of these 5'-terminal nucleotides must be G, whereas a less strict preference for a purine is evident at the third position, and A is slightly favored over G at the first position {{PubMed|23610425,23610407}}
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| * '''Protein family:''' [[Nudix hydrolase]] family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU30630&redirect=T BSU30630]
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| * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=4JZV 4JZV] {{PubMed|23610407}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O35013 O35013]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU30630]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[mutTA]]'' {{PubMed|9387221}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ytkD_3134983_3135459_-1 mutTA] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|14761999}}, [[SigF]] {{PubMed|14761999}}
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| * '''Regulation:'''
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| ** expressed during logarithmic growth ([[SigA]]) and early during sporulation in the forespore ([[SigF]]) {{PubMed|16497325}}
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| == Reviews ==
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| <pubmed> 22568516</pubmed>
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| == Original publications ==
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| <pubmed>16513759,16497325, 14761999, 19011023 9387221 15576788 21925382 23610425 23610407 </pubmed>
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| [[Category:Protein-coding genes]]
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