|
|
| (5 intermediate revisions by 2 users not shown) |
| Line 1: |
Line 1: |
| * '''Description:''' 8-amino-7-oxononanoate synthase <br/><br/>
| | {{#bank: BioF}} |
|
| |
|
| {| align="right" border="1" cellpadding="2"
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''bioF''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || 8-amino-7-oxononanoate synthase
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of biotin
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU30220 bioF]
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/biotin/index.html Biotin]'''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 42 kDa, 6.285
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1167 bp, 389 aa
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[bioD]]'', ''[[bioA]]''
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15000]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:bioF_context.gif]]
| |
| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
| |
| |-
| |
| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bioF_3092184_3093353_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:bioF_expression.png|500px]]
| |
| |-
| |
| |}
| |
|
| |
| __TOC__
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
|
| |
|
| |
| <br/><br/>
| |
|
| |
| = [[Categories]] containing this gene/protein =
| |
| {{SubtiWiki category|[[biosynthesis of cofactors]]}}
| |
|
| |
| = This gene is a member of the following [[regulons]] =
| |
| {{SubtiWiki regulon|[[BirA regulon]]}}
| |
|
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Locus tag:''' BSU30220
| |
|
| |
| ===Phenotypes of a mutant ===
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/bioWAFDBI-ytbQ.html]
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11527]
| |
|
| |
| === Additional information===
| |
| * A mutation was found in this gene after evolution under relaxed selection for sporulation {{PubMed|21821766}}
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:''' 6-carboxyhexanoyl-CoA + L-alanine = 8-amino-7-oxononanoate + CoA + CO<sub>2</sub> (according to Swiss-Prot)
| |
|
| |
| * '''Protein family:''' class-II pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)
| |
|
| |
| * '''Paralogous protein(s):'''
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:'''
| |
|
| |
| * '''Domains:'''
| |
|
| |
| * '''Modification:'''
| |
|
| |
| * '''Cofactor(s):'''
| |
|
| |
| * '''Effectors of protein activity:'''
| |
|
| |
| * '''[[SubtInteract|Interactions]]:'''
| |
|
| |
| * '''[[Localization]]:'''
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''Structure:'''
| |
|
| |
| * '''UniProt:''' [http://www.uniprot.org/uniprot/P53556 P53556]
| |
|
| |
| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU30220]
| |
|
| |
| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.3.1.47 2.3.1.47]
| |
|
| |
| === Additional information===
| |
|
| |
| =Expression and regulation=
| |
| * '''Operon:''' ''[[bioW]]-[[bioA]]-[[bioF]]-[[bioD]]-[[bioB]]-[[bioI]]-[[ytbQ]]'' {{PubMed|8763940,8892842}}
| |
|
| |
| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bioF_3092184_3093353_-1 bioF] {{PubMed|22383849}}
| |
|
| |
| * '''Sigma factor:'''
| |
|
| |
| * '''Regulation:'''
| |
| ** repressed in the presence of biotin ([[BirA]]) {{PubMed|8763940,8892842}}
| |
|
| |
| * '''Regulatory mechanism:'''
| |
| ** [[BirA]]: transcription repression {{PubMed|8763940,8892842}}
| |
|
| |
| * '''Additional information:'''
| |
|
| |
| =Biological materials =
| |
|
| |
| * '''Mutant:'''
| |
|
| |
| * '''Expression vector:'''
| |
|
| |
| * '''lacZ fusion:'''
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:'''
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| =Labs working on this gene/protein=
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
| ==Reviews: {{PubMed|21437340}}==
| |
|
| |
| ==Original publications==
| |
| <pubmed>8763940,8892842,21821766,22960854 </pubmed>
| |
|
| |
| [[Category:Protein-coding genes]]
| |
