|
|
| (8 intermediate revisions by 3 users not shown) |
| Line 1: |
Line 1: |
| * '''Description:''' translation initiation factor IF-3 <br/><br/>
| | {{#bank: InfC}} |
|
| |
|
| {| align="right" border="1" cellpadding="2"
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''infC''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || initiation factor IF-3
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || translation
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 10.342
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 519 bp, 173 aa
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rpmI]]'', ''[[yscA]]''
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14847]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:infC_context.gif]]
| |
| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
| |
| |-
| |
| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=infC_2952828_2953349_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:infC_expression.png|500px]]
| |
| |-
| |
| |}
| |
|
| |
| __TOC__
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
|
| |
|
| |
| <br/><br/>
| |
|
| |
| = [[Categories]] containing this gene/protein =
| |
| {{SubtiWiki category|[[translation]]}},
| |
| {{SubtiWiki category|[[essential genes]]}}
| |
|
| |
| = This gene is a member of the following [[regulons]] =
| |
| {{SubtiWiki regulon|[[RplT regulon]]}},
| |
| {{SubtiWiki regulon|[[stringent response]]}}
| |
|
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Locus tag:''' BSU28870
| |
|
| |
| ===Phenotypes of a mutant ===
| |
|
| |
| essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/infC-rpmI-rplT-ysdA.html]
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11944]
| |
|
| |
| === Additional information===
| |
|
| |
|
| |
|
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:'''
| |
|
| |
| * '''Protein family:''' IF-3 family (according to Swiss-Prot)
| |
|
| |
| * '''Paralogous protein(s):'''
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:'''
| |
|
| |
| * '''Domains:'''
| |
|
| |
| * '''Modification:'''
| |
|
| |
| * '''Cofactor(s):'''
| |
|
| |
| * '''Effectors of protein activity:'''
| |
|
| |
| * '''[[SubtInteract|Interactions]]:'''
| |
|
| |
| * '''[[Localization]]:'''
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1TIF 1TIF] (N-terminal domain, Geobacillus stearothermophilus), [http://www.rcsb.org/pdb/explore.do?structureId=1TIG 1TIG] (C-terminal domain, Geobacillus stearothermophilus)
| |
|
| |
| * '''UniProt:''' [http://www.uniprot.org/uniprot/P55872 P55872]
| |
|
| |
| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU28870]
| |
|
| |
| * '''E.C. number:'''
| |
|
| |
| === Additional information===
| |
|
| |
| =Expression and regulation=
| |
| * '''Operon:''' ''[[infC]]-[[rpmI]]-[[rplT]]-[[ysdA]]'' {{PubMed|17289755}}
| |
|
| |
| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=infC_2952828_2953349_-1 infC] {{PubMed|22383849}}
| |
|
| |
| * '''Sigma factor:''' [[SigA]] {{PubMed|17289755}}
| |
|
| |
| * '''Regulation:'''
| |
| ** [[RelA]] dependent downregulation (Class I) during stringent response {{PubMed|11948165}}
| |
|
| |
| * '''Regulatory mechanism:'''
| |
| ** binding of [[RplT]] to a [[RNA switch]] in the ''[[infC]]'' leader region causes transcription termination {{PubMed|17289755}}
| |
|
| |
| * '''Additional information:'''
| |
| ** expression of [[infC|IF3]] is uncoupled from that of [[rpmI|L35]] and [[rplT|L20]] by [[rny|RNase Y]]-dependent processing {{PubMed|21843271}}
| |
|
| |
| =Biological materials =
| |
|
| |
| * '''Mutant:'''
| |
|
| |
| * '''Expression vector:'''
| |
|
| |
| * '''lacZ fusion:'''
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:'''
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| =Labs working on this gene/protein=
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
| '''Additional publications:''' {{PubMed|21303765}}
| |
| <pubmed>17289755 11948165 21843271</pubmed>
| |
| [[Category:Protein-coding genes]]
| |
