Pyk - Revision history

Jstuelk at 17:13, 16 May 2014

2014-05-16T17:13:43Z

134.76.70.252 at 09:48, 17 April 2014

2014-04-17T09:48:21Z

← Older revision		Revision as of 09:48, 17 April 2014
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	* '''Additional information:'''		* '''Additional information:'''
	** belongs to the 100 [[most abundant proteins]] {{PubMed\|15378759}}		** belongs to the 100 [[most abundant proteins]] {{PubMed\|15378759}}
			** number of protein molecules per cell (minimal medium with glucose and ammonium): 2986 {{PubMed\|24696501}}
			** number of protein molecules per cell (complex medium with amino acids, without glucose): 11657 {{PubMed\|24696501}}

	=Biological materials =		=Biological materials =

134.76.38.147 at 14:29, 2 April 2014

2014-04-02T14:29:56Z

← Older revision		Revision as of 14:29, 2 April 2014
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	=== Database entries ===		=== Database entries ===
			* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29180&redirect=T BSU29180]

	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pfkA-pyk-ytzA.html]		* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pfkA-pyk-ytzA.html]
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	=== Database entries ===		=== Database entries ===
			* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29180&redirect=T BSU29180]

	* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2E28 2E28] (Geobacillus stearothermophilus)		* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2E28 2E28] (Geobacillus stearothermophilus)

Jstuelk at 14:43, 5 March 2014

2014-03-05T14:43:36Z

← Older revision		Revision as of 14:43, 5 March 2014
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	{{SubtiWiki category\|[[ATP synthesis]]}},		{{SubtiWiki category\|[[ATP synthesis]]}},
	{{SubtiWiki category\|[[carbon core metabolism]]}},		{{SubtiWiki category\|[[carbon core metabolism]]}},
	{{SubtiWiki category\|[[phosphoproteins]]}}		{{SubtiWiki category\|[[phosphoproteins]]}},
			[[most abundant proteins]]

	= This gene is a member of the following [[regulons]] =		= This gene is a member of the following [[regulons]] =
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	* '''Additional information:'''		* '''Additional information:'''
			** belongs to the 100 [[most abundant proteins]] {{PubMed\|15378759}}

	=Biological materials =		=Biological materials =
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	=References=		=References=

	<pubmed>17726680 16493705 11489127 17505547 10966427 17218307 3711058 4623707 21821766 22846916 23420519 24158146 24571712</pubmed>		<pubmed>17726680 16493705 11489127 17505547 10966427 17218307 3711058 4623707 21821766 22846916 23420519 24158146 24571712 15378759</pubmed>

	[[Category:Protein-coding genes]]		[[Category:Protein-coding genes]]

Jstuelk at 16:32, 28 February 2014

2014-02-28T16:32:41Z

← Older revision		Revision as of 16:32, 28 February 2014
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	=== Extended information on the protein ===		=== Extended information on the protein ===

	* '''Kinetic information:''' ~~Allosteric~~ Regulation [http://www.ncbi.nlm.nih.gov/pubmed/3711058 PubMed]		* '''Kinetic information:'''
			** allosteric Regulation [http://www.ncbi.nlm.nih.gov/pubmed/3711058 PubMed]
			** specific activity: 0.231 µmol min<sup>-1</sup> (mg protein)<sup>-1</sup> {{PubMed\|24571712}}

	* '''Domains:'''		* '''[[Domains]]:'''

	* '''Modification:''' phosphorylation on Ser36 [http://www.ncbi.nlm.nih.gov/pubmed/17218307 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/16493705 PubMed], phosphorylation on Ser536 or Ser546 [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed], please note that the Ser is not on position 536 but rather at 538		* '''Modification:''' phosphorylation on Ser36 [http://www.ncbi.nlm.nih.gov/pubmed/17218307 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/16493705 PubMed], phosphorylation on Ser536 or Ser546 [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed], please note that the Ser is not on position 536 but rather at 538

	* '''~~Cofactor(s)~~:''' Mg2+, K+		* '''[[Cofactors]]:''' Mg2+, K+

	* '''Effectors of protein activity:'''		* '''Effectors of protein activity:'''
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	=References=		=References=

	<pubmed>17726680 16493705 11489127 17505547 10966427 17218307 3711058 4623707 21821766 22846916 23420519 24158146 </pubmed>		<pubmed>17726680 16493705 11489127 17505547 10966427 17218307 3711058 4623707 21821766 22846916 23420519 24158146 24571712</pubmed>

	[[Category:Protein-coding genes]]		[[Category:Protein-coding genes]]

134.76.70.252 at 11:26, 7 January 2014

2014-01-07T11:26:36Z

← Older revision		Revision as of 11:26, 7 January 2014
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	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29180 pyk]		\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29180 pyk]
	\|-		\|-
	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/~~pathways~~/~~carbon_flow~~.~~html Central C-metabolism~~]'''		\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=pyk pyk]'''
	\|-		\|-
	\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 61,9 kDa, 4.88		\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 61,9 kDa, 4.88

Jstuelk: /* References */

2013-11-28T11:22:23Z

References

← Older revision		Revision as of 11:22, 28 November 2013
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	=References=		=References=

	<pubmed>17726680 16493705 11489127 17505547 10966427 17218307 3711058 4623707 21821766 22846916 23420519</pubmed>		<pubmed>17726680 16493705 11489127 17505547 10966427 17218307 3711058 4623707 21821766 22846916 23420519 24158146 </pubmed>

	[[Category:Protein-coding genes]]		[[Category:Protein-coding genes]]

Jstuelk at 16:02, 13 July 2013

2013-07-13T16:02:14Z

← Older revision		Revision as of 16:02, 13 July 2013
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	<br/><br/><br/><br/>		<br/><br/><br/><br/>
	<br/><br/><br/><br/>		<br/><br/><br/><br/>
	~~<br/><br/><br/><br/>~~		<br/><br/>
	~~<br/><br/><br/><br/>~~


	~~<br/><br/><br/><br/>~~<br/><br/>

	= [[Categories]] containing this gene/protein =		= [[Categories]] containing this gene/protein =
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	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pyk_2984788_2986545_-1 pyk] {{PubMed\|22383849}}		* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pyk_2984788_2986545_-1 pyk] {{PubMed\|22383849}}

	* '''Sigma factor:'''		* '''[[Sigma factor]]:'''

	* '''Regulation:'''		* '''Regulation:'''
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	* '''Mutant:'''		* '''Mutant:'''
	* GP589 (''pyk''::''cat''), available in [[Stülke]] lab		* GP589 (''pyk''::''cat''), available in [[Jörg Stülke]]'s lab, {{PubMed\|23420519}}
	* GP600 (''pyk''::''erm''), available in [[Stülke]] lab		* GP600 (''pyk''::''erm''), available in [[Jörg Stülke]]'s lab, {{PubMed\|23420519}}

	* '''Expression vector:'''		* '''Expression vector:'''
	** expression in ''E. coli'', N-terminal His-tag: pGP1100 (in [[pWH844]]), available in [[Stülke]] lab		** expression in ''E. coli'', N-terminal His-tag: pGP1100 (in [[pWH844]]), available in [[Jörg Stülke]]'s lab
	** expression in ''B. subtilis'', native protein: pGP1411 (in [[pBQ200]]), available in [[Stülke]] lab		** expression in ''B. subtilis'', native protein: pGP1411 (in [[pBQ200]]), available in [[Jörg Stülke]]'s lab
	** expression in ''B. subtilis'', N-terminal Strep-tag: pGP1409 (in [[pGP380]]), available in [[Stülke]] lab		** expression in ''B. subtilis'', N-terminal Strep-tag: pGP1409 (in [[pGP380]]), available in [[Jörg Stülke]]'s lab
	** expression in ''B. subtilis'', C-terminal Strep-tag: pGP1410 (in [[pGP382]]), available in [[Stülke]] lab		** expression in ''B. subtilis'', C-terminal Strep-tag: pGP1410 (in [[pGP382]]), available in [[Jörg Stülke]]'s lab

	* '''lacZ fusion:''' see ''[[pfkA]]''		* '''lacZ fusion:''' see ''[[pfkA]]''
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	* '''GFP fusion:'''		* '''GFP fusion:'''

	* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab		* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab

	* '''Antibody:'''		* '''Antibody:'''
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	=References=		=References=

	<pubmed>17726680 16493705 11489127 17505547 10966427 17218307 3711058 4623707 21821766 22846916 </pubmed>		<pubmed>17726680 16493705 11489127 17505547 10966427 17218307 3711058 4623707 21821766 22846916 23420519</pubmed>

	[[Category:Protein-coding genes]]		[[Category:Protein-coding genes]]

134.76.70.252 at 14:16, 16 May 2013

2013-05-16T14:16:04Z

← Older revision		Revision as of 14:16, 16 May 2013
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	<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>		<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
	\|-		\|-
	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pyk_2984788_2986545_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:pyk_expression.png\|500px]]		\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pyk_2984788_2986545_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:pyk_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29180]]
	\|-		\|-
	\|}		\|}

134.76.70.252 at 10:59, 14 May 2013

2013-05-14T10:59:25Z

← Older revision		Revision as of 10:59, 14 May 2013
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	\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[ytzA]]'', ''[[pfkA]]''		\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[ytzA]]'', ''[[pfkA]]''
	\|-		\|-
	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29180 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29180 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29180 ~~Advanced_DNA~~]		\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29180 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29180 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29180 DNA_with_flanks]
	\|-		\|-
	\|colspan="2" \| '''Genetic context''' <br/> [[Image:pyk_context.gif]]		\|colspan="2" \| '''Genetic context''' <br/> [[Image:pyk_context.gif]]