View source for MccB

* '''Description:''' cystathionine lyase/  homocysteine gamma-lyase <br/><br/>

{| align="right" border="1" cellpadding="2" 
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''mccB ''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yrhB''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cystathionine lyase/  homocysteine gamma-lyase 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || methionine-to-cysteine conversion
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27250 mccB]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=mccB mccB]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 5.215  
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1137 bp, 379 aa 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yrhC]]'', ''[[mccA]]''
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU27250 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU27250 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU27250 DNA_with_flanks]
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:yrhB_context.gif]]
 <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mccB_2785001_2786140_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:mccB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27250]]
|-
|}

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= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[biosynthesis/ acquisition of amino acids]]}},
{{SubtiWiki category|[[membrane proteins]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[CymR regulon]]}},
{{SubtiWiki regulon|[[Spx regulon]]}}

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU27250

===Phenotypes of a mutant ===

=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU27250&redirect=T BSU27250]

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yrrT-mtn-yrhABC.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12291]

=== Additional information===




=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' L-cystathionine + H<sub>2</sub>O = L-cysteine + NH<sub>3</sub> + 2-oxobutanoate (according to Swiss-Prot) 

* '''Protein family:''' trans-sulfuration enzymes family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''	

* '''Domains:''' 

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''

* '''[[Localization]]:'''
** cell membrane (according to Swiss-Prot)

=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU27250&redirect=T BSU27250]

* '''Structure:'''	

* '''UniProt:''' [http://www.uniprot.org/uniprot/O05394 O05394]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU27250]

* '''E.C. number:''' [http://www.expasy.org/enzyme/4.4.1.1 4.4.1.1]

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[yrrT]]-[[mtnN]]-[[mccA]]-[[mccB]]-[[yrhC]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/17056751 PubMed]

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mccB_2785001_2786140_-1 mccB] {{PubMed|22383849}}

* '''Sigma factor:''' [[SigA]] {{PubMed|16513748}}

* '''Regulation:''' 
** repressed in the presence of cysteine ([[CymR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/16513748 PubMed]

* '''Regulatory mechanism:''' 
** [[CymR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/16513748 PubMed]
** [[Spx]]: transcription activation {{PubMed|12642660,16885442}}

* '''Additional information:'''
** number of protein molecules per cell (minimal medium with glucose and ammonium): 164 {{PubMed|24696501}}

=Biological materials =

* '''Mutant:'''
** 1A942 ( ''mccB''::''kan''), {{PubMed|17056751}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A942&Search=1A942 BGSC]
** 1A945 ( ''mccB''::''kan''), {{PubMed|17056751}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A945&Search=1A945 BGSC]

* '''Expression vector:'''
        
* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:''' 

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>17056751,16513748,16885442, 12642660, </pubmed>

[[Category:Protein-coding genes]]