yitF

168

similar to mandelate racemase

Locus: BSU_10970

Molecular weight: 41.89 kDa

Isoelectric point: 7.53

Protein length: 371 aa

Gene length: 1116 bp

Function: unknown

Product: unknown

Essential: no

E.C.: null

Synonyms: yitF

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG4948 (Galperin et al., 2021)

Gene

Coordinates: 1,174,861 → 1,175,976

The protein

Protein family

• mandelate racemase/muconate lactonizing enzyme family (according to UniProt)

Structure

• 2GDQ (PDB)
• O06741 (AlphaFold)

Expression and Regulation

Operons

• Genes: yitG-yitF

  Description: PubMed

  Regulation

  • expressed during sporulation in the forespore (SigG) PubMed

  Sigma factors

  • SigG: sigma factor, PubMed, in sigG regulon

Biological materials

Mutant

• MGNA-B180 (yitF::erm), available at the NBRP B. subtilis, Japan
• BKE10970 (ΔyitF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CCGTACAATTTTCACACTGG,  downstream forward: _UP4_TAAGCTGGGCCATTTCTTTA
• BKK10970 (ΔyitF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CCGTACAATTTTCACACTGG,  downstream forward: _UP4_TAAGCTGGGCCATTTCTTTA

References

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