oapB

168

similar to OLE RNA binding protein OapB

Locus: BSU_01389

Molecular weight: 8.30 kDa

Isoelectric point: 8.932

Protein length: 74 aa

Gene length: 225 bp

Function: unknown

Product: unknown

Essential: -

E.C.: null

Synonyms: ybzG

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 147,312  147,536

The protein

Domains

• KOW domain (aa 7 - 60)

Structure

• 7K9B (PDB) (from B. halodurans, 41% identity) PubMed
• C0H3S8 (AlphaFold)

Expression and Regulation

Operons

• Genes: rpsJ-rplC-rplD-rplW-rplB-rpsS-rplV-rpsC-rplP-rpmC-rpsQ-rplN-rplX-rplE-rpsN-rpsH-rplF-rplR-rpsE-rpmD-rplO-secY-adk-map-oapB-infA-rpmJ-rpsM-rpsK-S56-rpoA-rplQ

  Description: PubMed

  Regulation

  • strongly repressed in response to glucose starvation in M9 medium PubMed
  • likely autorepression of operon expression upon binding of excess of one ribosomal protein to the untranslated region of the mRNA PubMed
  • expression is reduced in motile cells as compared to non-motile cells PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response

  Sigma factors

  • SigA: sigma factor, PubMed (two promoters, about 140bp and 200bp upstream of the start codon), in sigA regulon

  Additional information

  • term-seq has identified a potential novel regulatory RNA element (protein-dependent leader) including an intrinsic transcription terminator upstream of rpsJ PubMed

Biological materials

Mutant

• BKE01389 (oapB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGCTTGAAAAACATCTGT,  downstream forward: _UP4_TGAAATTTGTCAGAAAGCAA
• BKK01389 (oapB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGCTTGAAAAACATCTGT,  downstream forward: _UP4_TGAAATTTGTCAGAAAGCAA

References

Reviews

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Original Publications

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