xynD

168

arabinoxylan arabinofuranohydrolase

Locus: BSU_18160

Molecular weight: 54.33 kDa

Isoelectric point: 8.03

Protein length: 513 aa

Gene length: 1542 bp

Function: arabinoxylan degradation

Product: arabinoxylan arabinofuranohydrolase

Essential: no

E.C.: 3.2.1.55

Synonyms: xynD

Genomic Context

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List of homologs in different organisms, belongs to COG3507 (Galperin et al., 2021)

Gene

Coordinates: 1,944,113  1,945,654

The protein

Catalyzed reaction/ biological activity

• Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides (according to UniProt)

Protein family

• glycosyl hydrolase 43 family (according to UniProt)

Domains

• CBM6 domain (aa 382-511) (accordig to UniProt)

Structure

• 3C7O (PDB) PubMed
• Q45071 (AlphaFold)

Localization

• extracellular (signal peptide) PubMed

Expression and Regulation

Operons

• Genes: xynD-xynC

  Description: PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in abrB regulon

Biological materials

Mutant

• BKE18160 (xynD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCACTTCACATCCTCCTT,  downstream forward: _UP4_TAGTACTCGTAATGGGACCG
• BKK18160 (xynD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCACTTCACATCCTCCTT,  downstream forward: _UP4_TAGTACTCGTAATGGGACCG

References

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