ureB

168

urease (beta subunit)

Locus: BSU_36650

Molecular weight: 13.50 kDa

Isoelectric point: 9.75

Protein length: 124 aa

Gene length: 375 bp

Function: utilization of urea as alternative nitrogen source

Product: urease (beta subunit)

Essential: no

E.C.: 3.5.1.5

Synonyms: ureB

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0832 (Galperin et al., 2021)

Gene

Coordinates: 3,768,420 → 3,768,794

The protein

Catalyzed reaction/ biological activity

• 2 H⁺ + H₂O + urea --> CO₂ + 2 NH₄⁺ (according to UniProt)

Protein family

• urease beta subunit family (single member, according to UniProt)

Structure

• 3UBP (PDB) (from B. pasteurii, 48% identity) PubMed
• P71035 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ywzF-ywzE-ureA-ureB-ureC

  Description: PubMed

• Genes: ureA-ureB-ureC

  Description: PubMed

  Regulation

  • induced by nitrogen limitation (GlnR, TnrA) PubMed

  Regulatory mechanism

  • GlnR: repression, PubMed, in glnR regulon
  • TnrA: activation, PubMed, in tnrA regulon
  • PucR: activation, PubMed, in pucR regulon
  • CodY: repression, PubMed, in codY regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigH: sigma factor, PubMed, in sigH regulon

Biological materials

Mutant

• BKE36650 (ΔureB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AGCAATTTGAAATGCTCCCG,  downstream forward: _UP4_GGCTGGATGGAGGGTGTAAT
• BKK36650 (ΔureB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AGCAATTTGAAATGCTCCCG,  downstream forward: _UP4_GGCTGGATGGAGGGTGTAAT

References

Reviews

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Original Publications

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