tagA

168

UDP-N-acetyl-D-mannosamine transferase

Locus: BSU_35750

Molecular weight: 29.35 kDa

Isoelectric point: 10.13

Protein length: 256 aa

Gene length: 771 bp

Function: biosynthesis of teichoic acid

Product: UDP-N-acetyl-D-mannosamine transferase

Essential: yes

E.C.: 2.4.1.187

Synonyms: tagA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1922 (Galperin et al., 2021)

Gene

Coordinates: 3,681,370  3,682,140

Phenotypes of a mutant

• essential PubMed, reduced growth rate, loss of rod-like morphology PubMed
• inactivation of ''tagA strongly restores beta-lactam resistance in a sigM'' mutant PubMed
• knockdown of expression results in loss of ICEBs1 conjugation (both in donor and recipient cells) PubMed

The protein

Catalyzed reaction/ biological activity

• N-acetyl-α-D-glucosaminyl-1-diphospho-di-trans,octa-cis-undecaprenol + UDP-N-acetyl-α-D-mannosamine --> H⁺ + N-acetyl-β-D-mannosaminyl-(1→4)-N-acetyl-α-D-glucosaminyl di-trans,octa-cis-undecaprenyl diphosphate + UDP (according to UniProt)

Protein family

• glycosyltransferase 26 family (single member, according to UniProt)

Structure

• 5WB4 (PDB) (from Thermoanaerobacter italicus, corresponds to aa 35 ... 194, 33% identity)
• P27620 (AlphaFold)

Localization

• secreted (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: tagA-tagB

  Description: PubMed

  Regulation

  • repressed when phosphate is available (PhoP) PubMed
  • the leader mRNA is processed upstream of tagA by RNase Y, this requires the RicA-RicF-RicT complex PubMed

  Regulatory mechanism

  • PhoP: repression, PubMed, in phoP regulon
  • WalR: activation, PubMed, in walR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

References

Reviews

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Original Publications

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