ssuD

168

aliphatic sulfonate monooxygenase

Locus: BSU_08860

Molecular weight: 41.64 kDa

Isoelectric point: 5.37

Protein length: 376 aa

Gene length: 1131 bp

Function: sulfonate reduction

Product: aliphatic sulfonate monooxygenase

Essential: no

E.C.: null

Synonyms: ssuD, ygcA, yzeC

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2141 (Galperin et al., 2021)

Gene

Coordinates: 964,027  965,157

The protein

Catalyzed reaction/ biological activity

• alkanesulfonate + FMNH₂ + O₂ --> aldehyde + FMN + 2 H⁺ + H₂O + sulfite (according to UniProt)

Protein family

• ssuD family (single member, according to UniProt)

Structure

• 1NQK (PDB) (from Escherichia coli, 64% identity, 76% similarity)
• P40402 (AlphaFold)

Expression and Regulation

Operons

• Genes: ssuB-ssuA-ssuC-ssuD-ygaN

  Description: PubMed

  Regulation

  • repressed in the presence of cysteine (CymR) PubMed

  Regulatory mechanism

  • CymR: repression, PubMed, in cymR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-B466 (ygcA::erm), available at the NBRP B. subtilis, Japan
• 1A951 ( ssuD::cat), PubMed, available at BGSC
• 1A8083 ( ssuD::kan), PubMed, available at BGSC
• BKE08860 (ssuD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTCATCTCCCCTTTAT,  downstream forward: _UP4_TGAGTTTAACTAAAATACAG
• BKK08860 (ssuD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTCATCTCCCCTTTAT,  downstream forward: _UP4_TGAGTTTAACTAAAATACAG

References

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