rpsH

168

ribosomal protein uS8

Locus: BSU_01300

Molecular weight: 14.70 kDa

Isoelectric point: 10.3

Protein length: 132 aa

Gene length: 399 bp

Function: translation

Product: ribosomal protein S8 (uS8)

Essential: yes

Synonyms: rpsH

Genomic Context

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List of homologs in different organisms, belongs to COG0096 (Galperin et al., 2021)

Gene

Coordinates: 141,974 → 142,372

Phenotypes of a mutant

• essential PubMed

The protein

Protein family

• universal ribosomal protein uS8 family (single member, according to UniProt)

Structure

• 1SEI (PDB) (Geobacillus stearothermophilus)
• 3J9W (PDB) (the ribosome) PubMed
• P12879 (AlphaFold)

Modification

• phosphorylated on Arg-72 PubMed

Expression and Regulation

Operons

• Genes: rpsJ-rplC-rplD-rplW-rplB-rpsS-rplV-rpsC-rplP-rpmC-rpsQ-rplN-rplX-rplE-rpsN-rpsH-rplF-rplR-rpsE-rpmD-rplO-secY-adk-map-oapB-infA-rpmJ-rpsM-rpsK-S56-rpoA-rplQ

  Description: PubMed

  Regulation

  • strongly repressed in response to glucose starvation in M9 medium PubMed
  • likely autorepression of operon expression upon binding of excess of one ribosomal protein to the untranslated region of the mRNA PubMed
  • expression is reduced in motile cells as compared to non-motile cells PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response

  Sigma factors

  • SigA: sigma factor, PubMed (two promoters, about 140bp and 200bp upstream of the start codon), in sigA regulon

  Additional information

  • term-seq has identified a potential novel regulatory RNA element (protein-dependent leader) including an intrinsic transcription terminator upstream of rpsJ PubMed

References

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