rpsF

168

ribosomal protein bS6

Locus: BSU_40910

Molecular weight: 10.99 kDa

Isoelectric point: 4.99

Protein length: 95 aa

Gene length: 288 bp

Function: translation

Product: ribosomal protein bS6

Essential: no

Synonyms: rpsF

Genomic Context

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List of homologs in different organisms, belongs to COG0360 (Galperin et al., 2021)

Gene

Coordinates: 4,199,445 4,199,732

The protein

Protein family

• bacterial ribosomal protein bS6 family (single member, according to UniProt)

Structure

• 3R3T (PDB) (from Bacillus anthracis, 71% identity, 97% similarity)
• 3J9W (PDB) (the ribosome) PubMed
• P21468 (AlphaFold)

Additional information

• belongs to the 100 most abundant proteins PubMed
• this ribosomal protein is lacking in some organisms with very small genomes PubMed

Expression and Regulation

Operons

• Genes: yyzM-yyaE-yyaF-rpsF-ssbA-rpsR

  Description: PubMed

• Genes: yyaF-rpsF-ssbA-rpsR

  Description: PubMed

  Regulation

  • ComK: transcription activation PubMed

  Regulatory mechanism

  • ComK: activation, in comK regulon
  • stringent response: negative regulation, in stringent response

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: rpsF-ssbA-rpsR

  Description: PubMed

  Regulation

  • ComK: transcription activation PubMed

Biological materials

Mutant

• BKE40910 (rpsF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTGTTTGCACCTCCTTT, downstream forward: _UP4_TAAGCAATTTTGAAATATAT
• BKK40910 (rpsF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTGTTTGCACCTCCTTT, downstream forward: _UP4_TAAGCAATTTTGAAATATAT

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

References

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