rho

168

transcriptional termination protein, prevents the formation of antiterminator-like RNA structures that could otherwise compete with the terminator hairpin, involved in balanced regulation of cell motility, [category|SW.4.1.2|Biofilm formation], and [category|SW.4.2|Sporulation]

locus: BSU_37080

Molecular weight: 48.51 kDa

pI: 6.86

Protein length: 427 aa

Gene length: 1284 bp

function: transcriptional termination

product: transcriptional termination protein

essential: no

synonyms: rho

List of homologs in different organisms, belongs to COG1158 (Galperin et al., 2021)

Gene

Coordinates: 3,803,400  3,804,683

Phenotypes of a mutant

• loss of motility due to poor expression of the [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD] regulon, this can be partially reversed by inactivation of [gene|920F91E748EE079FF864011D9052B073567C41E4|slrR] [pubmed|28723971]
• no [category|SW.4.1.2|Biofilm formation], this can be suppressed by deletion of [gene|5A6FBAE6553343092862CB79E150F934978C32A9|sinR] and by switching of expression of the [gene|52A47B49B1FB955EE7E3C316B57A2B4134F78480|epsO]-[gene|F6C4634BF871D9A01E27014FFED7527C54BF560D|epsA] antisense transcript [pubmed|28723971]
• increased efficiency of [category|SW.4.2|Sporulation], due to enhanced [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A] phosphorylation [pubmed|28723971]
• defective in [category|SW.4.1.4|Swarming] motility [pubmed|35638827]

The protein

Catalyzed reaction/ biological activity

• prevents the formation of antiterminator-like RNA structures that could otherwise compete with the terminator hairpin [pubmed|36154805]

Protein family

• Rho family (single member, according to UniProt)

[wiki|Domains]

• [wiki|CSD domain] (aa 54-127) (according to the InterPro database)
• Rho RNA-BD domain (aa 51-125) (according to UniProt)

Structure

• [PDB|1PVO] (Rho from ''E. coli'') [Pubmed|12859904]
• [PDB|8HSR] ([protein|7CD46C64BE69EDADF58B634DC50C3F61BFFEC5B5|rho]-[wiki|RNA polymerase] termination complex from Thermus thermophilus) [pubmed|36753546]
• [AF|Q03222]

Expression and Regulation

Operons

• genes: [gene|9896B99346B0D3F6D57F57377DB253B46135A37A|spo0F]-[gene|800EC1F1CA8C4F2A643EDECBB684347C29CAB0CA|fbaA]-[gene|1419F2F99D924973D75EE6FAA41F9A27EC5283E1|ywjH]-[gene|523B01B5E7141C7DFC28CE67EB92580F49C20844|murAB]-[gene|0AFCE5C602B073B29DB15DB3CDD153E05E3D02BD|glpX]-[gene|7CD46C64BE69EDADF58B634DC50C3F61BFFEC5B5|rho]-[gene|035154A4160F084BA1CC124D0951937B203E167D|rpmE]

  description: [pubmed|39427753]

  expression

  • readthrough-transcription from the [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]-dependent promoter after the initiation of sporulation in the mother cell [pubmed|39427753]

• genes: [gene|7CD46C64BE69EDADF58B634DC50C3F61BFFEC5B5|rho]-[gene|035154A4160F084BA1CC124D0951937B203E167D|rpmE]

  description: [pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, during vegetative growth [pubmed|39427753], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, after initiation of sporulation in the forespore [pubmed|39427753], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]

Biological materials

Mutant

• GP2673 ([gene|7CD46C64BE69EDADF58B634DC50C3F61BFFEC5B5|rho]::''ermC''), available in [wiki|Jörg Stülke]'s lab
• BKE37080 ([gene|7CD46C64BE69EDADF58B634DC50C3F61BFFEC5B5|rho]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37080 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAAAAACACCACGCTTTT,  downstream forward: _UP4_TAAATTGTATTTGCAAAAGA
• BKK37080 ([gene|7CD46C64BE69EDADF58B634DC50C3F61BFFEC5B5|rho]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37080 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAAAAACACCACGCTTTT,  downstream forward: _UP4_TAAATTGTATTTGCAAAAGA

References

Reviews

• 16946247,12887917,16946247,8576105,23347833,12887917,23704790,26796109,28731845,29094196,36154805,40476722

Original Publications

• 36815589,11566991,10027981,20075920,22383849,12859904,21040729,25112476,28723971,26857544,29931073,30845912,33243850,34805793,35638827,36192538,36735730,36753546,39427753