pucD

168

xanthine dehydrogenase

Locus: BSU_32480

Molecular weight: 80.25 kDa

Isoelectric point: 5.75

Protein length: 745 aa

Gene length: 2238 bp

Function: purine utilization

Product: xanthine dehydrogenase

Essential: no

Synonyms: pucD, yurC

Genomic Context

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List of homologs in different organisms, belongs to COG1529 (Galperin et al., 2021)

Gene

Coordinates: 3,336,263 → 3,338,500

The protein

Catalyzed reaction/ biological activity

• H₂O + NAD⁺ + xanthine --> H⁺ + NADH + urate (according to UniProt)
• H₂O + hypoxanthine + NAD⁺ --> H⁺ + NADH + xanthine (according to UniProt)

Protein family

• xanthine dehydrogenase family (single member, according to UniProt)

Structure

• 1RM6 (PDB) (4-hydroxybenzoyl-CoA reductase from Thauera aromatica, 30% identity) PubMed
• O32144 (AlphaFold)

Expression and Regulation

Operons

• Genes: pucA-pucB-pucC-pucD-pucE

  Description: PubMed

  Regulation

  • induced in the presence of purine nucleotides (effector: allantoin) (PucR) PubMed

  Regulatory mechanism

  • PucR: activation, PubMed, in pucR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A964 (yurC::erm), available at the NBRP B. subtilis, Japan
• BKE32480 (ΔpucD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTATTCCCCTCCCTCAG,  downstream forward: _UP4_CAATGGACATAAAAGAGGCC
• BKK32480 (ΔpucD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTATTCCCCTCCCTCAG,  downstream forward: _UP4_CAATGGACATAAAAGAGGCC

References

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