phoB

168

alkaline phosphatase III, heptaprenylglyceryl phosphate processing phosphatase

Locus: BSU_05740

Molecular weight: 50.36 kDa

Isoelectric point: 5.89

Protein length: 462 aa

Gene length: 1389 bp

Function: aquisition of phosphate upon phosphate starvation, ether lipid synthesis

Product: alkaline phosphatase III, heptaprenylglyceryl phosphate processing phosphatase

Essential: no

E.C.: 3.1.3.1

Synonyms: phoB

Genomic Context

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List of homologs in different organisms, belongs to COG1785 (Galperin et al., 2021)

Gene

Coordinates: 620,097  621,485

The protein

Catalyzed reaction/ biological activity

• phosphate monoester + H₂O --> alcohol + phosphate (according to UniProt)

Protein family

• alkaline phosphatase family (with PhoA, according to UniProt)

Structure

• 3A52 (PDB) (from Shewanella sp., 41% identity) PubMed
• P19405 (AlphaFold)

Modification

• phosphorylated on ser/ thr/ tyr PubMed

Paralogous protein(s)

• PhoA

Localization

• extracellular (signal peptide) PubMed

Expression and Regulation

Operons

• Genes: phoB-ydhF

  Description: PubMed

  Regulation

  • expressed under conditions of phosphate limitation (PhoP) PubMed

  Regulatory mechanism

  • PhoP: activation, PubMed, in phoP regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon
  • SigA: sigma factor, in sigA regulon

Biological materials

Mutant

• 1A767 ( phoB::cat), PubMed, available at BGSC
• BKE05740 (phoB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACTGGATAACCTCCTAAA,  downstream forward: _UP4_TAAAAGAATACAAGGTGTCC
• BKK05740 (phoB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACTGGATAACCTCCTAAA,  downstream forward: _UP4_TAAAAGAATACAAGGTGTCC

References

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