pepT

168

peptidase T (tripeptidase), zinc-dependent

locus: BSU_38920

Molecular weight: 45.35 kDa

pI: 4.54

Protein length: 410 aa

Gene length: 1233 bp

function: peptide degradation

product: peptidase T (tripeptidase)

essential: no

ec: null

synonyms: pepT

List of homologs in different organisms, belongs to COG2195 (Galperin et al., 2021)

Gene

Coordinates: 3,995,075 → 3,996,307

The protein

Catalyzed reaction/ biological activity

• Release of the N-terminal residue from a tripeptide (according to UniProt)

Protein family

• peptidase M20B family (single member, according to UniProt)

Structure

• [PDB|3GBO] (from ''Bacillus cereus (71% identity''), to be published)
• [AF|P55179]

Paralogous protein(s)

• [protein|4D56C583BBAF47F384ACFACF7A607FD8BFA59960|yqjE]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|B77F24856B19A8101EDDB8A95D4E201F44C789DC|pepT]

  description: [Pubmed|10746760]

Biological materials

Mutant

• MGNA-B733 (pepT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1732 NBRP B. subtilis, Japan]
• BKE38920 (Δ[gene|B77F24856B19A8101EDDB8A95D4E201F44C789DC|pepT]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38920 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCCAATCATCTCCTTT,  downstream forward: _UP4_TAACGCCAAAAGCCAGTCCA
• BKK38920 (Δ[gene|B77F24856B19A8101EDDB8A95D4E201F44C789DC|pepT]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38920 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCCAATCATCTCCTTT,  downstream forward: _UP4_TAACGCCAAAAGCCAGTCCA

References

• 8978088,10987140