pbpX

168

Class C penicillin-binding protein X

Locus: BSU_16950

Molecular weight: 43.70 kDa

Isoelectric point: 9.71

Protein length: 391 aa

Gene length: 1176 bp

Function: endopeptidase

Product: penicillin-binding protein X

Essential: no

Synonyms: pbpX

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1680 (Galperin et al., 2021)

Gene

Coordinates: 1,765,859  1,767,034

Phenotypes of a mutant

• increased sensitivity to lysozyme PubMed
• inactivation of ''pbpX strongly restores beta-lactam resistance in a sigM'' mutant PubMed

The protein

Protein family

• beta-lactamase family (according to UniProt)

Structure

• 3TG9 (PDB) (a PBP from B. halodurans, aa 90-375, 28% identity)
• O31773 (AlphaFold)

Localization

• extracellular (signal peptide) PubMed
• during vegetative growth: septal, low flourescence at periphery PubMed
• during sporulation: both asymmetric septa and the prespore PubMed

Expression and Regulation

Operons

• Genes: pbpX

  Description: PubMed

  Regulation

  • expression is reduced in a SigV mutant PubMed

  Sigma factors

  • SigM: sigma factor, PubMed, in sigM regulon
  • SigX: sigma factor, PubMed, in sigX regulon
  • SigV: sigma factor, PubMed, in sigV regulon

Biological materials

Mutant

• MGNA-B074 (pbpX::erm), available at the NBRP B. subtilis, Japan
• BKE16950 (pbpX::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCATTTTTTCCTCCCAA,  downstream forward: _UP4_TGATTGGCGATCTTCTTTTG
• BKK16950 (pbpX::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCATTTTTTCCTCCCAA,  downstream forward: _UP4_TGATTGGCGATCTTCTTTTG

GFP fusion

• 3107 trpC2 pbpX::pSG5047 (cat P_xyl-gfpa-pbpX1-426) PubMed, available in Dirk Jan Scheffers' lab and in the BGSC

References

Reviews

Loading

Original Publications

Loading