narK

168

nitrite extrusion protein

Locus: BSU_37320

Molecular weight: 42.80 kDa

Isoelectric point: 10.1

Protein length: 395 aa

Gene length: 1188 bp

Function: nitrate respiration, nitrite export

Product: nitrite extrusion protein

Essential: no

Synonyms: narK

Genomic Context

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List of homologs in different organisms, belongs to COG2223 (Galperin et al., 2021)

Gene

Coordinates: 3,832,327 → 3,833,514

The protein

Protein family

• major facilitator superfamily (according to UniProt)
• Nitrate/nitrite porter (TC 2.A.1.8) family (with NasA, according to UniProt)

Structure

• 4JR9 (PDB) (from E. coli, 25% identity) PubMed
• P46907 (AlphaFold)

Paralogous protein(s)

• NasA

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: narK-fnr

  Description: PubMed

  Regulation

  • ''fnr'': expressed under anaerobic conditions (ResD) PubMed

  Regulatory mechanism

  • NsrR: repression, PubMed, in nsrR regulon
  • Fnr: activation, PubMed, in fnr regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE37320 (ΔnarK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGGCTTGCCCCTTTCA,  downstream forward: _UP4_TAACACTGGGGCATTCACAA
• BKK37320 (ΔnarK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGGCTTGCCCCTTTCA,  downstream forward: _UP4_TAACACTGGGGCATTCACAA

References

Reviews

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Original Publications

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