manP

168

mannose-specific permease of the phosphotransferase system, EIIBCA of the PTS, Trigger enzyme, control of ManR activity

Locus: BSU_12010

Molecular weight: 61.81 kDa

Isoelectric point: 6.72

Protein length: 650 aa

Gene length: 1953 bp

Function: mannose uptake and phosphorylation, control of ManR activity

Product: mannose-specific PTS, EIIBCA

Essential: no

Synonyms: manP, yjdD

Genomic Context

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List of homologs in different organisms, belongs to COG1762 (Galperin et al., 2021)

Gene

Coordinates: 1,272,725  1,274,677

The protein

Catalyzed reaction/ biological activity

• transport  and concomitant phosphorylation of mannose
• D-mannose + N^π-phospho-L-histidyl-[protein] --> D-mannose 6-phosphate + L-histidyl-[protein] (according to UniProt)

Protein family

• PTS permease, fructose/ mannitol family PubMed

Domains

• 3 PTS EIIC domains type-2 (aa 1-98, aa 123-456, aa 504-649) (according to UniProt)

Structure

• 2R48 (PDB) (IIA domain)
• 2R4Q (PDB) (FruA, IIB domain, 56% identity)
• O31645 (AlphaFold)

Modification

• phosphorylation on Ser-365 PubMed

Paralogous protein(s)

• FruA

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: manP-manA-yjdF

  Description: PubMed

  Regulation

  • induced by mannose (ManR) PubMed
  • expession may be controlled by a potential RNA switch located in the 5' untranslated region of the manP-manA-yjdF mRNA between manA and yjdF

  Regulatory mechanism

  • ManR: activation, in the presence of mannose and absence of glucose PubMed, in manR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A270 (yjdD::erm), available at the NBRP B. subtilis, Japan
• BKE12010 (manP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGGAAACCTCCTTTAAA,  downstream forward: _UP4_ATCGAATAAAGCGGGGGATT
• BKK12010 (manP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGGAAACCTCCTTTAAA,  downstream forward: _UP4_ATCGAATAAAGCGGGGGATT

References

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