hutP

168

transcriptional antiterminator of the hut operon

Locus: BSU_39340

Molecular weight: 16.43 kDa

Isoelectric point: 6.06

Protein length: 151 aa

Gene length: 456 bp

Function: regulation of histidine utilization

Product: transcriptional antiterminator

Essential: no

Synonyms: hutP, hutP1

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 4,041,483 → 4,041,938

The protein

Protein family

• hutP family (single member, according to UniProt)

Cofactors

• Mg²⁺ PubMed
• L-His PubMed

Structure

• 3BOY (PDB) (complex bound  to the hut mRNA),  1WPT (PDB)
• P10943 (AlphaFold)

Effectors of protein activity

• HutP binds its RNA target to cause antitermination in the presence of histidine PubMed

Expression and Regulation

Operons

• Genes: hutP-hutH-hutU-hutI-hutG-hutM

  Description: PubMed

  Regulation

  • induced by histidine (HutP) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon
  • CodY: repression, PubMed, in codY regulon
  • HutP: antitermination, at a protein-dependent RNA switch located between hutP and hutH PubMed, in hutP regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE39340 (ΔhutP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGAATCACTCAATTCC,  downstream forward: _UP4_TGAAACAGCCCATAGATCTT
• BKK39340 (ΔhutP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGAATCACTCAATTCC,  downstream forward: _UP4_TGAAACAGCCCATAGATCTT

References

Reviews

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Research papers

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