hisA

168

phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase, homologue to HisF

Locus: BSU_34880

Molecular weight: 26.38 kDa

Isoelectric point: 4.84

Protein length: 245 aa

Gene length: 738 bp

Function: biosynthesis of histidine

Product: phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase, homologue to HisF

Essential: no

E.C.: 5.3.1.16

Synonyms: hisA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0106 (Galperin et al., 2021)

Gene

Coordinates: 3,584,317  3,585,054

The protein

Catalyzed reaction/ biological activity

• 1-(5-phospho-β-D-ribosyl)-5-[(5-phospho-β-D-ribosylamino)methylideneamino]imidazole-4-carboxamide --> 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamide (according to UniProt)

Protein family

• HisA/HisF family (with HisF, according to UniProt)

Structure

• 2VEP (PDB) (from Streptomyces coelicolor, 41% identity, 58% similarity) PubMed
• O35006 (AlphaFold)

Paralogous protein(s)

• HisF

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: hisZ-hisG-hisD-hisB-hisH-hisA-hisF-hisI

  Description: PubMed

  Regulation

  • expression during spore germination is strongly reduced under conditions of osmotic stress PubMed
  • expression depends on functional RnpM PubMed

  Regulatory mechanism

  • RnpM: unknown, PubMed, in rnpM regulon

Biological materials

Mutant

• 1A626 ( hisA::erm), PubMed, available at BGSC
• BKE34880 (hisA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGCCGGATATAATGTAAAAG,  downstream forward: _UP4_GCGCTTGAAAGGGTGAAGCG
• BKK34880 (hisA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGCCGGATATAATGTAAAAG,  downstream forward: _UP4_GCGCTTGAAAGGGTGAAGCG

References

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