glnM

168

glutamine [wiki|ABC transporter] (membrane protein)

locus: BSU_27450

Molecular weight: 23.55 kDa

pI: 8.42

Protein length: 216 aa

Gene length: 651 bp

function: glutamine uptake

product: glutamine [wiki|ABC transporter] (membrane protein)

essential: no

synonyms: glnM

List of homologs in different organisms, belongs to COG0765 (Galperin et al., 2021)

Gene

Coordinates: 2,803,990  2,804,640

The protein

Protein family

• [wiki|Binding-protein-dependent transport system permease family] (according to UniProt)

[wiki|Domains]

• [wiki|ABC transmembrane type-1 domain] (aa 17-205) (according to UniProt)

Structure

• [PDB|4YMS] (complex with [protein|CD32011A08697B2C121996A02F1BD7BE4F932778|glnP]; from ''Thermoanaerobacter tengcongensis '', 40% identity) [Pubmed|25848002]
• [AF|O34671]

Paralogous protein(s)

• [protein|CD32011A08697B2C121996A02F1BD7BE4F932778|glnP]

[wiki|Localization]

• cell membrane [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|468BEA9EAEA589D683DB79B2C689A5C6A22AB67F|glnQ]-[gene|BAB70547672C855AEB3A4D4624044340CF582848|glnH]-[gene|6CE6AFDBE85974B45041DE924A09CC9F56ED79D8|glnM]-[gene|CD32011A08697B2C121996A02F1BD7BE4F932778|glnP]

  description: [Pubmed|12823818]

  regulation

  • expressed during sporulation ([protein|search|SigE]) [Pubmed|15699190]

  regulatory mechanism

  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: activation, [Pubmed|12823818], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]

Biological materials

Mutant

• BKE27450 ([gene|6CE6AFDBE85974B45041DE924A09CC9F56ED79D8|glnM]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27450 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTGCCTATTCACTCCTT,  downstream forward: _UP4_AGCTATTGATAAGGAGTGAG
• BKK27450 ([gene|6CE6AFDBE85974B45041DE924A09CC9F56ED79D8|glnM]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27450 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTGCCTATTCACTCCTT,  downstream forward: _UP4_AGCTATTGATAAGGAGTGAG

References

• 36815589,10092453,25755103,12823818,15699190,25848002,20023025,39397791