fbp

168

fructose-1,6-bisphosphatase, involved in gluconeogenesis

Locus: BSU_40190

Molecular weight: 77.80 kDa

Isoelectric point: 5.6

Protein length: 671 aa

Gene length: 2016 bp

Function: gluconeogenesis

Product: fructose-1,6-bisphosphatase

Essential: no

E.C.: 3.1.3.11

Synonyms: fbp, fdp, yydE

Genomic Context

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List of homologs in different organisms, belongs to COG3855 (Galperin et al., 2021)

Gene

Coordinates: 4,128,029  4,130,044

The protein

Catalyzed reaction/ biological activity

• β-D-fructose 1,6-bisphosphate + H₂O --> β-D-fructose 6-phosphate + phosphate (according to UniProt)

Protein family

• FBPase class 3 family (single member, according to UniProt)

Structure

• Q45597 (AlphaFold)

Expression and Regulation

Operons

• Genes: fbp

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-B811 (yydE::erm), available at the NBRP B. subtilis, Japan
• BKE40190 (fbp::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAACCGTCCATCCCCTTT,  downstream forward: _UP4_TGAGGGGTGTGGGGTTCAGG
• BKK40190 (fbp::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAACCGTCCATCCCCTTT,  downstream forward: _UP4_TGAGGGGTGTGGGGTTCAGG

References

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