cysS

168

cysteine-tRNA synthetase

Locus: BSU_00940

Molecular weight: 53.74 kDa

Isoelectric point: 5.07

Protein length: 466 aa

Gene length: 1401 bp

Function: translation

Product: cysteine-tRNA synthetase

Essential: yes

E.C.: 6.1.1.16

Synonyms: cysS, snpA

Genomic Context

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List of homologs in different organisms, belongs to COG0215 (Galperin et al., 2021)

Gene

Coordinates: 113,450 → 114,850

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + L-cysteine + tRNA^Cys --> AMP + diphosphate + L-cysteinyl-tRNA^Cys (according to UniProt)

Protein family

• Class-I aminoacyl-tRNA synthetase family (according to UniProt)

Structure

• 8QHP (PDB) (from Pyrococcus furiosus, 48.6% identity) PubMed
• 1U0B (PDB) (from Escherichia coli, complex with tRNA^Cys 42% identity, 56% similarity) PubMed
• Q06752 (AlphaFold)

Modification

• phosphorylation on Ser-270 PubMed

Localization

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• Genes: gltX-cysE-cysS-mrnC-yacO-rae1

  Description: PubMed

  Regulation

  • expression transiently increases in the forespore PubMed
  • the T-box RNA is degraded by RNase Y PubMed

  Regulatory mechanism

  • T-box: RNA switch, overlaps a transcription terminator upstream of cysE, in T-box

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

References

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