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2026-11-09T14:11:32Z
User contributions
MediaWiki 1.43.11
https://subtiwiki.uni-goettingen.de/wiki/index.php?title=BsrF&diff=104772
BsrF
2009-07-14T16:57:50Z
<p>SabineBrantl: /* Biological materials */</p>
<hr />
<div>* '''Description:''' small RNA <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''bsrF''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''SR2 ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || small RNA<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length''' || bp<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yobO]]'', ''[[csaA]]''<br />
|-<br />
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/genE.txt Gene sequence (+200bp) ]''' <br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:genE_context.gif]]<br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br />
<br />
<br/><br/><br/><br/><br/><br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:'''<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' no entry<br />
<br />
* '''SubtiList entry:''' no entry<br />
<br />
* '''Structure:''' <br />
<br />
=== Additional information===<br />
<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''bsrF'' [http://www.ncbi.nlm.nih.gov/sites/entrez/19542274 PubMed]<br />
<br />
* '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19542274 PubMed]<br />
<br />
* '''Regulation:''' weakly activated by [[CodY]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19542274 PubMed]<br />
<br />
* '''Regulatory mechanism:''' [[CodY]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/19542274 PubMed]<br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutants:''' DB104::Delta bsrF::cat, DB104::Delta bsrF::ery, 168::Delta bsrF::cat, 168::Delta bsrF::ery available in the [[Sabine Brantl]] lab<br />
<br />
* '''Expression vectors:''' pWSR2, multi-copy vector for tetracycline-inducible expression of bsrF (kanamycin resistance gene)<br />
pUCB2SR2, multi-copy vector for constitutive expression of bsrF under its own promoter (kanamycin resistance gene) available in the [[Sabine Brantl]] lab<br />
<br />
* '''lacZ fusions:''' pACG1 to pACG19 containing regions of different size upstream of the bsrF promoter available in the [[Sabine Brantl]] lab<br />
<br />
=Labs working on this gene/RNA=<br />
<br />
* [[Sabine Brantl |Sabine Brantl]], Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany [http://www.uni-jena.de/AG_Bakteriengenetik_(_Doz__Dr__Sabine_Brantl_).html homepage]<br />
* Kouji Nakamura, Tsukuba, University, Japan<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
<pubmed>18948176, 19542274 </pubmed></div>
SabineBrantl
https://subtiwiki.uni-goettingen.de/wiki/index.php?title=BsrF&diff=104771
BsrF
2009-07-14T16:56:18Z
<p>SabineBrantl: /* Biological materials */</p>
<hr />
<div>* '''Description:''' small RNA <br/><br/><br />
<br />
{| align="right" border="1" cellpadding="2" <br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Gene name'''<br />
|''bsrF''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''SR2 ''<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Essential''' || no <br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Product''' || small RNA<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown<br />
|-<br />
|style="background:#ABCDEF;" align="center"| '''Gene length''' || bp<br />
|-<br />
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yobO]]'', ''[[csaA]]''<br />
|-<br />
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/genE.txt Gene sequence (+200bp) ]''' <br />
|-<br />
|colspan="2" | '''Genetic context''' <br/> [[Image:genE_context.gif]]<br />
|-<br />
|}<br />
<br />
__TOC__<br />
<br />
<br/><br/><br />
<br />
<br/><br/><br/><br/><br/><br />
=The gene=<br />
<br />
=== Basic information ===<br />
<br />
* '''Locus tag:'''<br />
<br />
===Phenotypes of a mutant ===<br />
<br />
=== Database entries ===<br />
<br />
* '''DBTBS entry:''' no entry<br />
<br />
* '''SubtiList entry:''' no entry<br />
<br />
* '''Structure:''' <br />
<br />
=== Additional information===<br />
<br />
<br />
=Expression and regulation=<br />
<br />
* '''Operon:''' ''bsrF'' [http://www.ncbi.nlm.nih.gov/sites/entrez/19542274 PubMed]<br />
<br />
* '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19542274 PubMed]<br />
<br />
* '''Regulation:''' weakly activated by [[CodY]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19542274 PubMed]<br />
<br />
* '''Regulatory mechanism:''' [[CodY]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/19542274 PubMed]<br />
<br />
* '''Additional information:'''<br />
<br />
=Biological materials =<br />
<br />
* '''Mutants:''' DB104::Delta bsrF::cat, DB104::Delta bsrF::ery, 168::Delta bsrF::cat, 168::Delta bsrF::ery<br />
<br />
* '''Expression vectors:''' pWSR2, multi-copy vector for tetracycline-inducible expression of bsrF (kanamycin resistance gene)<br />
pUCB2SR2, multi-copy vector for constitutive expression of bsrF under its own promoter (kanamycin resistance gene)<br />
<br />
* '''lacZ fusions:''' pACG1 to pACG19 containing regions of different size upstream of the bsrF promoter available in the [[Sabine Brantl]] lab<br />
<br />
=Labs working on this gene/RNA=<br />
<br />
* [[Sabine Brantl |Sabine Brantl]], Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany [http://www.uni-jena.de/AG_Bakteriengenetik_(_Doz__Dr__Sabine_Brantl_).html homepage]<br />
* Kouji Nakamura, Tsukuba, University, Japan<br />
<br />
=Your additional remarks=<br />
<br />
=References=<br />
<br />
<pubmed>18948176, 19542274 </pubmed></div>
SabineBrantl