yxjG

168

putative B12-independent methionine synthase

Locus: BSU_38960

Molecular weight: 42.96 kDa

Isoelectric point: 5.01

Protein length: 378 aa

Gene length: 1137 bp

Function: unknown

Product: putative methionine synthase

Essential: no

Synonyms: yxjG

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0620 (Galperin et al., 2021)

Gene

Coordinates: 3,999,350  4,000,486

The protein

Structure

• 1YPX (PDB) (the enzyme from Listeria monocytogenes, 49% identity)
• P42318 (AlphaFold)

Modification

• S-bacillithiolation upon hypochlorite stress on Cys-346 PubMed

Paralogous protein(s)

• YxjH

Expression and Regulation

Operons

• Genes: yxjG

  Description: PubMed

  Regulation

  • induced by methionine starvation (S-box) PubMed
  • the S-box RNA is degraded by RNase Y PubMed

  Regulatory mechanism

  • S-box: RNA switch, , the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed, in S-box

Biological materials

Mutant

• MGNA-B731 (yxjG::erm), available at the NBRP B. subtilis, Japan
• BKE38960 (yxjG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTGTCCATTCCTTCTT,  downstream forward: _UP4_TAAGCAAAGAAAAAAACACA
• BKK38960 (yxjG::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTGTCCATTCCTTCTT,  downstream forward: _UP4_TAAGCAAAGAAAAAAACACA

References

Loading