Difference between revisions of "YxeI"

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|style="background:#ABCDEF;" align="center"|'''Function''' || resistance to penicillin
 
|style="background:#ABCDEF;" align="center"|'''Function''' || resistance to penicillin
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU39540 yxeI]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU39540 yxeI]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 5.276   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 5.276   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yxeJ]]'', ''[[yxeH]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yxeJ]]'', ''[[yxeH]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15990&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU39540 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU39540 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU39540 Advanced_DNA]
 
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Revision as of 14:15, 13 May 2013

  • Description: penicillin acylase

Gene name yxeI
Synonyms
Essential no
Product penicillin acylase
Function resistance to penicillin
Gene expression levels in SubtiExpress: yxeI
MW, pI 37 kDa, 5.276
Gene length, protein length 984 bp, 328 aa
Immediate neighbours yxeJ, yxeH
Sequences Protein DNA Advanced_DNA
Genetic context
YxeI context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YxeI expression.png


























Categories containing this gene/protein

Resistance against toxins/ antibiotics

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU39540

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: acylation of penicillin V PubMed
  • Protein family: peptidase C59 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
    • the apparent K(m) values for penicillin V and the synthetic substrate 2-nitro-5-(phenoxyacetamido)-benzoic acid was 40mM and 0.63mM, respectively PubMed
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Priya Rathinaswamy, Sushama M Gaikwad, C G Suresh, Asmita A Prabhune, James A Brannigan, Guy G Dodson, Archana V Pundle
Purification and characterization of YxeI, a penicillin acylase from Bacillus subtilis.
Int J Biol Macromol: 2012, 50(1);25-30
[PubMed:21978958] [WorldCat.org] [DOI] (I p)

Kyle N Erwin, Shunji Nakano, Peter Zuber
Sulfate-dependent repression of genes that function in organosulfur metabolism in Bacillus subtilis requires Spx.
J Bacteriol: 2005, 187(12);4042-9
[PubMed:15937167] [WorldCat.org] [DOI] (P p)

Ken-Ichi Yoshida, Izumi Ishio, Eishi Nagakawa, Yoshiyuki Yamamoto, Mami Yamamoto, Yasutaro Fujita
Systematic study of gene expression and transcription organization in the gntZ-ywaA region of the Bacillus subtilis genome.
Microbiology (Reading): 2000, 146 ( Pt 3);573-579
[PubMed:10746760] [WorldCat.org] [DOI] (P p)