pgcM

168

beta-phosphoglucomutase/ glucose-1-phosphate phosphodismutase

Locus: BSU_34550

Molecular weight: 24.95 kDa

Isoelectric point: 4.76

Protein length: 226 aa

Gene length: 681 bp

Function: starch and maltodextrin utilization

Product: beta-phosphoglucomutase(EC 5.4.2.6 and EC 2.7.1.41)

Essential: no

E.C.: 5.4.2.6

Synonyms: pgcM, yvdM

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0637 (Galperin et al., 2021)

Gene

Coordinates: 3,546,873 → 3,547,553

The protein

Catalyzed reaction/ biological activity

• β-D-glucose 1-phosphate --> β-D-glucose 6-phosphate (according to UniProt)

Protein family

• HAD superfamily (according to UniProt)
• CbbY/CbbZ/Gph/YieH family (with YhcW, according to UniProt)

Structure

• 3NAS (PDB)
• O06995 (AlphaFold)

Paralogous protein(s)

• YhdA, (31%)
  YhcW, (31%)

Expression and Regulation

Operons

• Genes: mdxR-yvdF-mdxE-mdxF-mdxG-yvdJ-yvdK-malL-pgcM

  Description: PubMed

  Regulation

  • induced in the presence of starch, β-cyclomaltodextrin, or maltose PubMed

  Regulatory mechanism

  • MdxR: activation, PubMed, in mdxR regulon

Biological materials

Mutant

• MGNA-B622 (yvdM::erm), available at the NBRP B. subtilis, Japan
• BKE34550 (ΔpgcM::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTCTAAATCAAAAATGACCG,  downstream forward: _UP4_TGAAATGAAAAAAACCTGCA
• BKK34550 (ΔpgcM::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTCTAAATCAAAAATGACCG,  downstream forward: _UP4_TGAAATGAAAAAAACCTGCA

References

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