psdR

168

two-component response regulator (OmpR family), induction of psdA-psdB in response to lipid II-binding lantibiotics (nisin and gallidermin) and internal antimicrobial peptides (SdpC, SkfA)

Locus: BSU_34720

Molecular weight: 27.37 kDa

Isoelectric point: 4.68

Protein length: 237 aa

Gene length: 714 bp

Function: resistance against toxic peptides

Product: two-component response regulator (OmpR family)

Essential: no

Synonyms: psdR, yvcP

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0745 (Galperin et al., 2021)

Gene

Coordinates: 3,567,422  3,568,135

The protein

Protein family

• OmpR family of two-component response regulators

Domains

• Response regulatory domain (aa 3-116) (according to UniProt)

Structure

• 5DCL (PDB) (from Streptococcus agalactiae, 42% identity) PubMed
• O06978 (AlphaFold)

Modification

• phosphorylated by PsdS on an Asp residue

Effectors of protein activity

• phosphorylation likely affects DNA-binding activity

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: psdR-psdS-psdA-psdB

  Description: PubMed

  Regulation

  • ''psdA'': induced by nisin, gallidermin (PsdR) PubMed

  Regulatory mechanism

  • PsdR: activation, PubMed, in psdR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-B637 (yvcP::erm), available at the NBRP B. subtilis, Japan
• BKE34720 (psdR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGTTGATCCCCCTTATAT,  downstream forward: _UP4_GTCAATCGGAAGGATGAAGC
• BKK34720 (psdR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGTTGATCCCCCTTATAT,  downstream forward: _UP4_GTCAATCGGAAGGATGAAGC

References

Reviews

Loading

Original Publications

Loading