mnmL

168

radical SAM enzyme, synthesis of mnm⁵s²U at the Wobble base position (U34) in tRNAs

Locus: BSU_30480

Molecular weight: 36.61 kDa

Isoelectric point: 5.61

Protein length: 322 aa

Gene length: 969 bp

Function: tRNA modification

Product: unknown

Essential: no

Synonyms: ytqA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1242 (Galperin et al., 2021)

Gene

Coordinates: 3,119,999  3,120,967

The protein

Catalyzed reaction/ biological activity

• 5-carboxymethylaminomethyl-2- thiouridine --> 5-aminomethyl-(2-thio)uridine PubMed
• synthesis of mnm⁵s²U at the Wobble base position (U34) in tRNAs PubMed

Protein family

• Radical SAM superfamily PubMed

Cofactors

• 4 Fe- 4S cluster PubMed

Structure

• O35008 (AlphaFold)

Expression and Regulation

Operons

• Genes: mnmL-mnmM

  Description: PubMed

Biological materials

Mutant

• MGNA-A129 (ytqA::erm), available at the NBRP B. subtilis, Japan
• BKE30480 (mnmL::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAATTCATGTGCCTCCTT,  downstream forward: _UP4_CGGCTTGAGGAGGAATCAGC
• BKK30480 (mnmL::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAATTCATGTGCCTCCTT,  downstream forward: _UP4_CGGCTTGAGGAGGAATCAGC

References

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