ytlP

168

similar to 2´-CPDase

Locus: BSU_29960

Molecular weight: 21.00 kDa

Isoelectric point: 8.5

Protein length: 183 aa

Gene length: 552 bp

Function: nucleotide metabolism

Product: putative 2´-CPDase

Essential: no

Synonyms: ytlP

Genomic Context

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List of homologs in different organisms, belongs to COG1514 (Galperin et al., 2021)

Gene

Coordinates: 3,065,751  3,066,302

The protein

Catalyzed reaction/ biological activity

• H₂O + RNA_(n)-2',3'-cyclic phosphate --> H⁺ + RNA_(n)-2'- phosphate (according to UniProt)

Protein family

• 2H phosphoesterase superfamily (with YjcG, according to UniProt) PubMed

Structure

• 1IUH (PDB) (from Thermus thermophilus, 24% identity) PubMed
• O34570 (AlphaFold)

Paralogous protein(s)

• YjcG

Expression and Regulation

Operons

• Genes: ytlP-ytlQ-ytlR-amyX

  Description: PubMed

  Sigma factors

  • SigD: sigma factor, PubMed, in sigD regulon

Biological materials

Mutant

• MGNA-A431 (ytlP::erm), available at the NBRP B. subtilis, Japan
• BKE29960 (ytlP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCGTCTGACACTCCCTT,  downstream forward: _UP4_TAATTCATTGCGGAGTGGAG
• BKK29960 (ytlP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCGTCTGACACTCCCTT,  downstream forward: _UP4_TAATTCATTGCGGAGTGGAG

References

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