yrdF

168

similar to ribonuclease inhibitor

locus: BSU_26730

Molecular weight: 10.59 kDa

pI: 3.94

Protein length: 91 aa

Gene length: 276 bp

function: unknown

product: unknown

essential: no

synonyms: yrdF

List of homologs in different organisms, belongs to COG2732 (Galperin et al., 2021)

Gene

Coordinates: 2,730,560 → 2,730,835

The protein

Protein family

• barstar family (single member, according to UniProt)

Structure

• [PDB|1A19] (from Bacillus amyloliquefaciens 49%identity)
• [AF|O07938]

Biological materials

Mutant

• BKE26730 (Δ[gene|C3BE9FD1560F1949A8096DDBA60632CF62F2D4F1|yrdF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE26730 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTAGAAACTATGCCCTCC,  downstream forward: _UP4_TAGACTTTCTGTTTTGCGAA
• BKK26730 (Δ[gene|C3BE9FD1560F1949A8096DDBA60632CF62F2D4F1|yrdF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK26730 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTAGAAACTATGCCCTCC,  downstream forward: _UP4_TAGACTTTCTGTTTTGCGAA

lacZ fusion

• pGP3807 (in [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab [pubmed|36377869]

References

• 27766092