yqgP

168

intramembrane protease

Locus: BSU_24870

Molecular weight: 56.27 kDa

Isoelectric point: 5.51

Protein length: 507 aa

Gene length: 1524 bp

Function: cell division

Product: intramembrane protease

Essential: no

Synonyms: yqgP, glpG, gluP

Genomic Context

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List of homologs in different organisms, belongs to COG5010 (Galperin et al., 2021)

Gene

Coordinates: 2,571,907  2,573,430

Phenotypes of a mutant

• filamentous phenotype PubMed

The protein

Catalyzed reaction/ biological activity

• Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains (according to UniProt)
• cleavage of MgtE between its first and second trans-membrane helix at low magnesium concentration or high manganese or zinc concentrations to prevent uptake of toxic metal ions PubMed
• acts as adapter protein for FtsH PubMed

Protein family

• peptidase S54 family (according to UniProt)

Domains

• two tetratrichopeptide repeats (according to UniProt)

Structure

• P54493 (AlphaFold)

Effectors of protein activity

• Mn²⁺ and Zn²⁺ bind to the N-terminal cytosolic domain of YqgE to stimulate MgtE degradation PubMed

Paralogous protein(s)

• YdcA, Array

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: yqgP-yqgO-glcK-yqgS

  Description: PubMed

  Additional information

  • PhoP∼P binds to the promoter region, but regulation has not been explored PubMed

Biological materials

Mutant

• MGNA-C445 (yqgP::erm), available at the NBRP B. subtilis, Japan
• BKE24870 (yqgP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTACCGCTCCAATAG,  downstream forward: _UP4_TAGAGGTGGAATATGGAAAG
• BKK24870 (yqgP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTACCGCTCCAATAG,  downstream forward: _UP4_TAGAGGTGGAATATGGAAAG

References

Reviews

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Original Publications

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