fni

168

isopentenyl diphosphate isomerase, last (8th) step in the MEP pathway of isoprenoid biosynthesis

Locus: BSU_22870

Molecular weight: 22.45 kDa

Isoelectric point: 6.1

Protein length: 349 aa

Gene length: 1050 bp

Function: MEP pathway of isoprenoid biosynthesis

Product: isopentenyl diphosphate isomerase

Essential: no

E.C.: 5.3.3.2

Synonyms: fni, ypgA, idi

Genomic Context

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List of homologs in different organisms, belongs to COG1304 (Galperin et al., 2021)

Gene

Coordinates: 2,393,602 → 2,394,651

The protein

Catalyzed reaction/ biological activity

• isopentenyl diphosphate --> dimethylallyl diphosphate (according to UniProt)

Protein family

• IPP isomerase type 2 family (single member, according to UniProt)

Structure

• 1P0N (PDB) (complex with FMN)
• 1P0K (PDB)
• P50740 (AlphaFold)

Localization

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• Genes: cmk-rpsA-fni

  Description: PubMed

• Genes: rpsA-fni

  Description: PubMed

• Genes: motI-ypfB-cmk-rpsA-fni

  Description: PubMed

Biological materials

Mutant

• MGNA-A420 (ypgA::erm), available at the NBRP B. subtilis, Japan
• BKE22870 (Δfni::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGCTCGAGTCACGTTTATCA,  downstream forward: _UP4_TAAATGATAAAAGCCCAAAA
• BKK22870 (Δfni::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGCTCGAGTCACGTTTATCA,  downstream forward: _UP4_TAAATGATAAAAGCCCAAAA

References

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