ypdQ

168

similar to RNase HI

Locus: BSU_21990

Molecular weight: 14.53 kDa

Isoelectric point: 5.62

Protein length: 132 aa

Gene length: 399 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: ypdQ

Genomic Context

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List of homologs in different organisms, belongs to COG0328 (Galperin et al., 2021)

Gene

Coordinates: 2,310,419  2,310,817

The protein

Protein family

• RNase H family (single member, according to UniProt)

Domains

• RNase H domain (aa 1-127) (according to UniProt)

Structure

• 1GOC (PDB) (RNase HI from E. coli, 30% identity) PubMed
• P54162 (AlphaFold)

Expression and Regulation

Operons

• Genes: ypdP-ypdQ

  Description: PubMed

Biological materials

Mutant

• MGNA-A871 (ypdQ::erm), available at the NBRP B. subtilis, Japan
• BKE21990 (ypdQ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTCTACATATATTTCTGTAG,  downstream forward: _UP4_TAAATCTTGATCATTGGTTC
• BKK21990 (ypdQ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTCTACATATATTTCTGTAG,  downstream forward: _UP4_TAAATCTTGATCATTGGTTC

References

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