ypbE

168

peptidoglycan-binding protein, likely involved in sidewall synthesis during cell growth

Locus: BSU_23000

Molecular weight: 27.14 kDa

Isoelectric point: 5.43

Protein length: 240 aa

Gene length: 723 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: ypbE

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 2,405,620 → 2,406,342

The protein

Domains

• contains a N-acetylglucosamine-polymer-binding LysM domain (aa 189-235) PubMed
• LysM domain (aa 189-235) (according to UniProt)

Structure

• P50731 (AlphaFold)

Effectors of protein activity

• binding to peptidoglycan is stimulated by YpbE multimerisation, induced by hexameric GpsB PubMed

Localization

• cell membrane PubMed

Biological materials

Mutant

• MGNA-A395 (ypbE::erm), available at the NBRP B. subtilis, Japan
• BKE23000 (ΔypbE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTCCGCCAATGCGGCGTTTT,  downstream forward: _UP4_TAGGGTATTATTTTTATTTT
• BKK23000 (ΔypbE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTCCGCCAATGCGGCGTTTT,  downstream forward: _UP4_TAGGGTATTATTTTTATTTT

References

Reviews

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Research papers

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