pghZ

168

phage-derived gamma polyglutamic acid hydrolase

Locus: BSU_20460

Molecular weight: 30.21 kDa

Isoelectric point: 8.59

Protein length: 274 aa

Gene length: 825 bp

Function: polyglutamic acid degradation

Product: phage-derived gamma polyglutamic acid hydrolase

Essential: no

Synonyms: pghZ, yoqZ

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG4195 (Galperin et al., 2021)

Gene

Coordinates: 2,189,961  2,190,785

The protein

Catalyzed reaction/ biological activity

• hydrolysis of polyglutamic acid PubMed

Protein family

• UPF0714 family (according to UniProt)

Domains

• DUF867 x, [[gamma-PGA hydrolase domain]] PubMed

Structure

• 3A9L (PDB) (from B. subtilis phage NIT1, 43% identity) PubMed
• O31913 (AlphaFold)

Paralogous protein(s)

• PghB, PghC, PghL

Biological materials

Mutant

• BKE20460 (pghZ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATCAACAACTCTCCTTAA,  downstream forward: _UP4_TATTGTATGGCTGGTGTAGC
• BKK20460 (pghZ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATCAACAACTCTCCTTAA,  downstream forward: _UP4_TATTGTATGGCTGGTGTAGC

References

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