rluD

168

pseudouridylate synthase, required for modification of 23S rRNA at positions 1940, 1944, and 1946

Locus: BSU_15460

Molecular weight: 33.50 kDa

Isoelectric point: 5.43

Protein length: 303 aa

Gene length: 912 bp

Function: 23S rRNA modification

Product: pseudouridylate synthase

Essential: no

E.C.: 5.4.99.23

Synonyms: ylyB, ylmL

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0564 (Galperin et al., 2021)

Gene

Coordinates: 1,617,210 → 1,618,121

The protein

Catalyzed reaction/ biological activity

• uridine in helix 69 of 23S rRNA (positions 1940, 1944, 1946) --> pseudouridine in RNA PubMed

Protein family

• S4 RNA-binding domain superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
• pseudouridine synthase RluA family (with YjbO and YhcT, according to UniProt)

Domains

• S4 RNA-binding domain (aa 15-74) (according to UniProt)

Structure

• 1V9F (PDB) (RluD from E. coli, 42% identical) PubMed
• Q45480 (AlphaFold)

Paralogous protein(s)

• YhcT, YjbO

Expression and Regulation

Operons

• Genes: lspA-rluD-pyrR

  Description: PubMed

Biological materials

Mutant

• BKE15460 (ΔrluD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AGCGGTTATATCAATTTGAT,  downstream forward: _UP4_TGACAGAGGGTTTCTTTTCT
• BKK15460 (ΔrluD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AGCGGTTATATCAATTTGAT,  downstream forward: _UP4_TGACAGAGGGTTTCTTTTCT

References

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