QueE

• Description: 7-carboxy-7-deazaguanine (CDG) synthase, required for the synthesis of the modified ribonucleotide queuosine
  
  

Gene name	queE
Synonyms	ykvL
Essential	no
Product	7-carboxy-7-deazaguanine (CDG) synthase
Function	tRNA modification
Gene expression levels in SubtiExpress: queE
Interactions involving this protein in SubtInteract: QueE
MW, pI	26 kDa, 4.962
Gene length, protein length	729 bp, 243 aa
Immediate neighbours	queD, queF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation

This gene is a member of the following regulons

preQ1 riboswitch

The gene

Basic information

• Locus tag: BSU13740

Database entries

• BsubCyc: BSU13740

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• • conversion of 6-carboxy-5,6,7,8-tetrahydropterin to 7-carboxy-7-deazaguanine (CDG) PubMed

• Catalyzed reaction/ biological activity:

• Protein family: radical S-adenosyl-L-methionine (SAM) superfamily

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): [4Fe-4S] cluster, S-adenosyl-L-methionine, Mg(2+) PubMed

• Effectors of protein activity:

• Interactions:
  • QueE-YkuN (electron transfer) PubMed

• Localization:

Database entries

• BsubCyc: BSU13740

• Structure:

• UniProt: O31677

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: queC-queD-queE-queF PubMed

• Expression browser: queE PubMed

• Sigma factor:

• Regulation:
  • repressed in the presence of queuosine (preQ1 riboswitch) PubMed

• Regulatory mechanism:
  • preQ1 riboswitch: transcriptional antitermination in the absence of queuosine PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: