ykgB

168

6-phosphogluconolactonase, general stress protein

Locus: BSU_13010

Molecular weight: 38.26 kDa

Isoelectric point: 5.15

Protein length: 349 aa

Gene length: 1050 bp

Function: pentose phosphate pathway

Product: 6-phosphogluconolactonase

Essential: no

E.C.: 3.1.1.31

Synonyms: ykgB, pgl

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2706 (Galperin et al., 2021)

Gene

Coordinates: 1,369,876  1,370,925

The protein

Catalyzed reaction/ biological activity

• 6-phosphogluconolactone - 6-phosphogluconate (pentose phosphate pathway) PubMed

Protein family

• cycloisomerase 2 family (single member, according to UniProt)

Structure

• 3HFQ (PDB) (from Lactobacillus plantarum, 42% identity)
• O34499 (AlphaFold)

Expression and Regulation

Operons

• Genes: ykgA-ykgB

  Description: PubMed

  Regulation

  • induced by stress (SigB) PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in sigB regulon

Biological materials

Mutant

• MGNA-A754 (ykgB::erm), available at the NBRP B. subtilis, Japan
• BKE13010 (ykgB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCGATTTCCCCCTTAAA,  downstream forward: _UP4_TAAACAAATGGAGTGGCTCT
• BKK13010 (ykgB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCGATTTCCCCCTTAAA,  downstream forward: _UP4_TAAACAAATGGAGTGGCTCT

FLAG-tag construct

• GP1402 (spc, based on pGP1331), available in Jörg Stülke's lab

References

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