forE1

168

essential subunit of formate:menadione oxidoreductase ForC1-ForE1, general stress protein, survival of ethanol and paraquat stresses

Locus: BSU_12170

Molecular weight: 21.13 kDa

Isoelectric point: 6.72

Protein length: 186 aa

Gene length: 561 bp

Function: survival of stress conditions

Product: partner subunit of formate:menadione oxidoreductase ForC1-ForE1

Essential: no

E.C.: null

Synonyms: yjgD

Genomic Context

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List of homologs in different organisms, belongs to COG2427 (Galperin et al., 2021)

Gene

Coordinates: 1,288,541  1,289,101

The protein

Protein family

• DUF1641 family (with YjlC and ForE2)

Structure

• O34681 (AlphaFold)

Expression and Regulation

Operons

• Genes: forC1-forE1

  Description: PubMed

  Regulation

  • induced by stress (SigB) PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in sigB regulon

Biological materials

Mutant

• MGNA-A377 (yjgD::erm), available at the NBRP B. subtilis, Japan
• BKE12170 (forE1::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GATTTTTTGGATTCGTTTAA,  downstream forward: _UP4_TGACCTCCCACCCGAGCGGC
• BKK12170 (forE1::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GATTTTTTGGATTCGTTTAA,  downstream forward: _UP4_TGACCTCCCACCCGAGCGGC

References

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