yhcY

168

two-component sensor kinase

Locus: BSU_09320

Molecular weight: 41.83 kDa

Isoelectric point: 6.64

Protein length: 379 aa

Gene length: 1140 bp

Function: unknown

Product: two-component sensor kinase

Essential: no

Synonyms: yhcY

Genomic Context

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List of homologs in different organisms, belongs to COG4585 (Galperin et al., 2021)

Gene

Coordinates: 1,008,668  1,009,807

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of YhcZ in a Asp residue
• ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

Domains

• Histidine kinase domain (aa 185-373) (according to UniProt)

Structure

• O07527 (AlphaFold)

Modification

• autophosphorylation on a His residue

Localization

• cytoplasm (Homogeneous) PubMed

Expression and Regulation

Operons

• Genes: yhcY-yhcZ-yhdA

  Description: PubMed

  Regulatory mechanism

  • LiaR: activation, PubMed, in liaR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A729 (yhcY::erm), available at the NBRP B. subtilis, Japan
• BKE09320 (yhcY::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGTTTCTCACTCCGGCAT,  downstream forward: _UP4_AAAAGCCGAAAAGGAGGGGC
• BKK09320 (yhcY::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGTTTCTCACTCCGGCAT,  downstream forward: _UP4_AAAAGCCGAAAAGGAGGGGC

References

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