yhaO

168

similar to DNA double-strand break repair protein

Locus: BSU_09910

Molecular weight: 46.65 kDa

Isoelectric point: 5.23

Protein length: 408 aa

Gene length: 1227 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yhaO, sbcF

Genomic Context

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List of homologs in different organisms, belongs to COG0420 (Galperin et al., 2021)

Gene

Coordinates: 1,064,846  1,066,072

Phenotypes of a mutant

• enhanced formation of rpsB or rpsE suppressor mutants after mitomycin treatment PubMed

The protein

Protein family

• Metallophosphoesterase superfamily (according to UniProt)

Structure

• 4TUG (PDB) (from Methanocaldococcus jannaschii, corresponds to aa 5 - 290, 27% identity) PubMed
• O07522 (AlphaFold)

Localization

• cytoplasm (Homogeneous) PubMed

Expression and Regulation

Operons

• Genes: yhaO-sbcE-yhaM

  Description: PubMed

  Regulation

  • induced by DNA damage (LexA) PubMed

  Regulatory mechanism

  • LexA: repression, PubMed, in lexA regulon

Biological materials

Mutant

• MGNA-A664 (yhaO::erm), available at the NBRP B. subtilis, Japan
• BKE09910 (yhaO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTGCTCCTTTCCAGGA,  downstream forward: _UP4_CTTAAGGTGCTTGATACATG
• BKK09910 (yhaO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTGCTCCTTTCCAGGA,  downstream forward: _UP4_CTTAAGGTGCTTGATACATG

References

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