yfjO

168

similar to ribosomal RNA methyltransferase

Locus: BSU_08020

Molecular weight: 51.97 kDa

Isoelectric point: 9.81

Protein length: 466 aa

Gene length: 1401 bp

Function: unknown

Product: putative rRNA methyltransferase

Essential: no

Synonyms: yfjO

Genomic Context

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List of homologs in different organisms, belongs to COG2265 (Galperin et al., 2021)

Gene

Coordinates: 873,402  874,802

The protein

Protein family

• Methyltransferase superfamily (according to UniProt)
• class I-like SAM-binding methyltransferase superfamily (according to UniProt)

Domains

• TRAM domain (aa 11-69) (according to UniProt)

Cofactors

• Fe-S cluster PubMed

Structure

• 5XJ1 (PDB) (from Streptococcus pneumoniae, 35% identity) PubMed
• O31545 (AlphaFold)

Paralogous protein(s)

• RlmCD

Expression and Regulation

Operons

• Genes: yfjP-yfjO

  Description: PubMed

Biological materials

Mutant

• MGNA-C276 (yfjO::erm), available at the NBRP B. subtilis, Japan
• BKE08020 (yfjO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGTTGTTCTCTCCGTTTC,  downstream forward: _UP4_TAAAAAGAACATTTCCCAGT
• BKK08020 (yfjO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGTTGTTCTCTCCGTTTC,  downstream forward: _UP4_TAAAAAGAACATTTCCCAGT

References

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