dnmA

168

DNA methyltransferase, forms N6-methyladenosine at nonpalindromic 5′-GACGAG-3′ sites

Locus: BSU_06760

Molecular weight: 100.96 kDa

Isoelectric point: 5.67

Protein length: 879 aa

Gene length: 2640 bp

Function: genome-wide N6-methyladenosine modifications at non-palindromic recognition sites to control promoter activities

Product: DNA methyltransferase

Essential: no

Synonyms: dnmA, yeeA

Genomic Context

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List of homologs in different organisms, belongs to COG1002 (Galperin et al., 2021)

Gene

Coordinates: 740,288 → 742,927

Additional information

• the dmnA-yeeB-yeeC gene cluster is a remnant of a phage defense system that no longer protects against phage predation PubMed

The protein

Catalyzed reaction/ biological activity

• formation of m6A DNA modifications at non-palindromic sites PubMed

Structure

• 5HR4 (PDB) (from Methylophilus methylotrophus, 38% identity) PubMed
• O31504 (AlphaFold)

Localization

• replisome region and the nucleoid PubMed

Expression and Regulation

Operons

• Genes: dgkB-rlmCD-yefC-dnmA-yeeB-yeeC

  Description: PubMed

Biological materials

Mutant

• MGNA-A959 (yeeA::erm), available at the NBRP B. subtilis, Japan
• BKE06760 (ΔdnmA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCGTTTCCCCCATTAA,  downstream forward: _UP4_ACAGAACGGTAGGTGTAAGG
• BKK06760 (ΔdnmA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCGTTTCCCCCATTAA,  downstream forward: _UP4_ACAGAACGGTAGGTGTAAGG

References

Research papers

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