yckE

168

aryl--glucosidase

Locus: BSU_03410

Molecular weight: 54.97 kDa

Isoelectric point: 5.57

Protein length: 477 aa

Gene length: 1434 bp

Function: utilization of aryl--glucosides

Product: aryl--glucosidase

Essential: no

E.C.: 3.2.1.86

Synonyms: yckE, bglC

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2723 (Galperin et al., 2021)

Gene

Coordinates: 370,259  371,692

The protein

Catalyzed reaction/ biological activity

• 6-phospho-β-D-glucosyl-(1→4)-D-glucose + H₂O --> D-glucose + D-glucose 6-phosphate (according to UniProt)

Protein family

• glycosyl hydrolase 1 family (according to UniProt)

Structure

• 4ZE4 (PDB) (from Geobacillus stearothermophilus, 72% identity)
• P42403 (AlphaFold)

Expression and Regulation

Operons

• Genes: yckE

  Description: PubMed

Biological materials

Mutant

• MGNA-C055 (yckE::erm), available at the NBRP B. subtilis, Japan
• BKE03410 (yckE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGTGTTTATCACTCCTG,  downstream forward: _UP4_TAAAGAGTCCCTGAGAGTTA
• BKK03410 (yckE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGTGTTTATCACTCCTG,  downstream forward: _UP4_TAAAGAGTCCCTGAGAGTTA

References

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