Difference between revisions of "YciC"

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|style="background:#ABCDEF;" align="center"|'''Function''' || zinc uptake
 
|style="background:#ABCDEF;" align="center"|'''Function''' || zinc uptake
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU03360 yciC]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU03360 yciC]
 
|-
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 4.399   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 4.399   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yczL]]'', ''[[yckA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yczL]]'', ''[[yckA]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12130&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU03360 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU03360 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU03360 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:yciC_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yciC_context.gif]]

Revision as of 12:07, 13 May 2013

  • Description: putative metallochaperone

Gene name yciC
Synonyms
Essential no
Product putative metallochaperone
Function zinc uptake
Gene expression levels in SubtiExpress: yciC
MW, pI 45 kDa, 4.399
Gene length, protein length 1191 bp, 397 aa
Immediate neighbours yczL, yckA
Sequences Protein DNA Advanced_DNA
Genetic context
YciC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YciC expression.png
























Categories containing this gene/protein

trace metal homeostasis (Cu, Zn, Ni, Mn, Mo), membrane proteins, phosphoproteins

This gene is a member of the following regulons

Zur regulon

The gene

Basic information

  • Locus tag: BSU03360

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: cobW C-terminal domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • phosphorylated on Arg-58 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
    • yciA: Zur: transcription repression in the presence of zinc PubMed
    • yciC: Zur: transcription repression in the presence of zinc PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

John Helmann, Cornell University, USA Homepage

Your additional remarks

References

Alexander K W Elsholz, Kürsad Turgay, Stephan Michalik, Bernd Hessling, Katrin Gronau, Dan Oertel, Ulrike Mäder, Jörg Bernhardt, Dörte Becher, Michael Hecker, Ulf Gerth
Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis.
Proc Natl Acad Sci U S A: 2012, 109(19);7451-6
[PubMed:22517742] [WorldCat.org] [DOI] (I p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Scott E Gabriel, Faith Miyagi, Ahmed Gaballa, John D Helmann
Regulation of the Bacillus subtilis yciC gene and insights into the DNA-binding specificity of the zinc-sensing metalloregulator Zur.
J Bacteriol: 2008, 190(10);3482-8
[PubMed:18344368] [WorldCat.org] [DOI] (I p)

Ahmed Gaballa, Tao Wang, Rick W Ye, John D Helmann
Functional analysis of the Bacillus subtilis Zur regulon.
J Bacteriol: 2002, 184(23);6508-14
[PubMed:12426338] [WorldCat.org] [DOI] (P p)

A Gaballa, J D Helmann
Identification of a zinc-specific metalloregulatory protein, Zur, controlling zinc transport operons in Bacillus subtilis.
J Bacteriol: 1998, 180(22);5815-21
[PubMed:9811636] [WorldCat.org] [DOI] (P p)