Difference between revisions of "XlyA"

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|style="background:#ABCDEF;" align="center"|'''Function''' || [[PBSX prophage]]-mediated lysis
 
|style="background:#ABCDEF;" align="center"|'''Function''' || [[PBSX prophage]]-mediated lysis
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU12810 xlyA]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU12810 xlyA]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 31 kDa, 5.342   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 31 kDa, 5.342   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[xhlB]]'', ''[[spoIISB]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[xhlB]]'', ''[[spoIISB]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13138&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU12810 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU12810 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU12810 Advanced_DNA]
 
|-
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:xlyA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:xlyA_context.gif]]

Revision as of 12:37, 13 May 2013

  • Description: N-acetylmuramoyl-L-alanine amidase

Gene name xlyA
Synonyms
Essential no
Product N-acetylmuramoyl-L-alanine amidase
Function PBSX prophage-mediated lysis
Gene expression levels in SubtiExpress: xlyA
MW, pI 31 kDa, 5.342
Gene length, protein length 891 bp, 297 aa
Immediate neighbours xhlB, spoIISB
Sequences Protein DNA Advanced_DNA
Genetic context
XlyA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
XlyA expression.png
























Categories containing this gene/protein

cell wall degradation/ turnover, PBSX prophage

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU12810

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)
  • Protein family: LysM repeat (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Lieh Yoon Low, Chen Yang, Marta Perego, Andrei Osterman, Robert Liddington
Role of net charge on catalytic domain and influence of cell wall binding domain on bactericidal activity, specificity, and host range of phage lysins.
J Biol Chem: 2011, 286(39);34391-403
[PubMed:21816821] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

S Krogh, S T Jørgensen, K M Devine
Lysis genes of the Bacillus subtilis defective prophage PBSX.
J Bacteriol: 1998, 180(8);2110-7
[PubMed:9555893] [WorldCat.org] [DOI] (P p)

P F Longchamp, C Mauël, D Karamata
Lytic enzymes associated with defective prophages of Bacillus subtilis: sequencing and characterization of the region comprising the N-acetylmuramoyl-L-alanine amidase gene of prophage PBSX.
Microbiology (Reading): 1994, 140 ( Pt 8);1855-67
[PubMed:7921239] [WorldCat.org] [DOI] (P p)