vmlR

168

translation factor, antibiotic resistance subfamily F ABC ATPase, allosterically dissociates antibiotics (virginiamycin M, lincomycin) from their ribosomal binding sites

Locus: BSU_05610

Molecular weight: 62.57 kDa

Isoelectric point: 6.73

Protein length: 547 aa

Gene length: 1644 bp

Function: dissociation of antibiotics (virginiamycin M, lincomycin) from the ribosome

Product: ABCF ATPase

Essential: no

Synonyms: vmlR, expZ

Genomic Context

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List of homologs in different organisms, belongs to COG0488 (Galperin et al., 2021)

Gene

Coordinates: 604,736  606,379

The protein

Protein family

• ABC transporter superfamily (according to UniProt)
• ABCF ATPase subfamily PubMed

Domains

• 2 ABC transporter domains (aa 5-200, aa 292-504) (according to UniProt)

Structure

• 6HA8 (PDB) PubMed
• P39115 (AlphaFold)

Localization

• cytoplasm PubMed

Expression and Regulation

Operons

• Genes: vmlR

  Description: PubMed

  Regulation

  • induced by virginiamycin M or lincomycin due to antibiotic-induced ribosome stalling during translation of an upstream open reading frame in the vmlR leader region resulting in transcription anti termination PubMed
  • NusG-dependent RNA polymerase pausing in the vmlR leader prevents leaky expression in the absence of antibiotic PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • A ncRNA is predicted between 'VmlR' and 'YdgF' PubMed

Biological materials

Mutant

• MGNA-C159 (expZ::erm), available at the NBRP B. subtilis, Japan
• BKE05610 (vmlR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCATATCCCTCGCTTTA,  downstream forward: _UP4_TGAGGGAGAAAGTTCAAGCG
• BKK05610 (vmlR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCATATCCCTCGCTTTA,  downstream forward: _UP4_TGAGGGAGAAAGTTCAAGCG

References

Reviews

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Original Publications

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